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文献和实验siRNA (RNA Oligo) Annealing Protocol
mammalian cells. Nature, 2001,411[6836]:494-8. Elbashir SM, Lendeckel W, Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev, 2001, 15[2]:188-200 Tuschl T, Zamore PD, Lehmann R, Bartel DP, Sharp PA. Targeted mRNA
3% TREVIGEL PROTOCOL for 3 bp resolution
with lid of bottle loose. Add dH2O to bring the volume above 400 ml and heat the gel in the microwave again until enough water has boiled off to bring the volume back to 400 ml. Place the bottle in sink and quickly degas with a water
Fill bottom of humid chamber with 100 ml 1X SSC. Prop slide between two tip boxes with lamp overhead and etch corners of array. Place arrays face down over 1X SSC and cover chamber with lid. Rehydrate until array spots glisten, approximately 5-15
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