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- Beckman Coulter贝克曼库尔特
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- 2025年07月13日
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文献和实验Construction and Characterization of Adenovirus Vectors
complexes to form for 15 min at room temperature. 18. Add 2.4 mL of MEM to each tube containing the DNA complexes. Mix well by gentle pipeting. 19. Rinse four 35-mm plates seeded with 293 cells (as described in Step 14
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to 37°C and 65°C Sonicator Spectrophotometer Thermal cycler, automated Timer Tubes, microcentrifuge, 1.5-mL Tubes, polypropylene, 14-mL (e.g., 17- x 100-mm) for use in high-speed centrifuge Tubes, polypropylene, 50-mL
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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