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文献和实验; HOWEVER, MORE THAN A FEW SECONDS OF EXPOSURE RESULTS IN CROSS-LINKING AND NO AMPLIFICATION 7. Use core samples as substrate in PCR reactions: I make up 40ul/reaction of reaction mix, and allow 10ul per core. Simply add core to mix, vortex a little, spin down, cover with mineral
CORE SAMPLE PCR: A method to re-PCR unique bands from products of mixed
of reaction mix, and allow 10ul per core. Simply add core to mix, vortex a little, spin down, cover with mineral oil. PCR according to taste (not inhibited by presence of a little bromophenol blue or of 50ng/ml ethidium bromide). 8. At end of PCR
also, cover port with foil Plug compressor and upper section of fermentor in Make sure drain tube is in sink cup in the hood and turn the water on Running I. Drive and temperature control Make sure heat and drive is off and speed
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