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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; optional wash after step 12 REQUIRED1.Centrifuge the PAXgene Blood RNA Tube for 10 min at 30005000 x g using a swing-out rotor.Note: Ensure that the blood sample
and then probing the library for the correct DNA can be extraordinarily tedious. I have applied a simple “core sampling”procedure - involving “coring” an agarose sample out of a gel, and using it as template for another round of PCR - to get around this problem
RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE
at 4o C, centrifuge for 10 mins at 1000 xg, transfer suppernatant to new tube, and store at -80o C 2. Add an equal volume of PCI and vortex for 2-5 mins (tubes can be placed on a shaker at 4o C up to 2 hours while extracting
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