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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
agitation to 250 RPM. Adjust air regulator on front of fermentor to 20 PSIG. Open the grey flowmeter valve to check that air is flowing into the growth chamber. Set airflow at ~10 using the flowmeter valve. Leave valve open during sterilization
Protocols for ET recombination
should be at OD600 of 0.3-0.4. Harvest. make sure the centrifuge and SA600 or SS34 (Sorvall) rotor is very cold by centrifuging for 10 min, -5℃ at 4,000 rpm. Spin 35 mls cells for 10 min at 7,000 rpm at -5℃. Put the other 35 mls on ice. Pour away
Protocols for ET recombination
oC at 4,000 rpm. （8）Spin 35 mls cells for 10 min at 7,000 rpm at -5oC. Put the other 35 mls on ice. （9）Pour away the supernatant, add the second 35 mls and respin. （10）Pour away supernatant, put tube on ice, resuspend cells
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