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文献和实验Reagents: NGS-Normal Goat Serum PBST-0.5% Tween-20/CMF-PBS CMF-PBS 1°Ab-mouse MF20 (anti-myosin monoclonal) 1°Ab-rabbit myoD (anti-myoD polyclonal) 2°Ab-goat anti-mouse IgG-FITC 2°Ab-goat anti-rabbit IgG-FITC
【转贴】immunofluorescence staining
off the parafilm. Sealing the edge of the dish with tape or parafilm during incubation, plus placing a small wad of wetted kim-wipe in the dish, will keep the coverslips from drying out. Note: This protocol has been optimized for HeLa cells, and works
96‐well PCR microplate (compatible with the qPCR machine) Transparent microplate sealing foil (compatible with the qPCR machine
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