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文献和实验Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method
of the membrane filter method ( 3 ). Brain slices are placed on a porous membrane filter that provides a suitable substratum and allows a thin film of media to cover the surface of the slice by capillary action (Fig. 1 ). The membrane inserts are placed
of interest, and the selected wavelengths can be changed based on the sensing requirements. We use AOTFs made of KDP, MgF2 , and TeO2 , with a Si-based CCD camera to cover different spectral regions from the UV to the NIR. A liquid crystal variable retarder (LCVR) is used
Cytokine induced angiogenesis in chick embryos.
cells that cover the vessels (Gilbert, 2000) In the first of two experiments, the VEGF and bFGF growth factors were used to induce angiogenesis; both are applied to the CAM membrane of the chick embryo. In the second experiment, only the effect
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