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文献和实验Yeast IF with Methanol/Acetone Dehydration
the wells of teflon coated slides with 0.1 polylysine (>400,000 MW) in water for 10 min at RT. Spin this solution for 10 min in a microfuge immedietly before use and stay away from the bottom. In general do high speed spins of all solutions
Yeast IF with Methanol/Acetone Dehydration
part of the procedure and unfortunately it is the most critical as well. Wash the cells once in sorbitol buffer and suspend in 100-500ul of the same. BE VERY VERY GENTLE! Place on ice. Coat the wells of teflon coated slides with 0.1% polylysine (>400,000 MW
on the bottom of the tube. Centrifuge the tubes at 40,000 xg for 60 minutes. Using the resulting nuclear pellet is just above the dense sucrose layer. It is used to extract chromatin proteins. Carefully remove the supernatant above the pellet
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