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文献和实验High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up
. For more information, see http://www.invitrogen.com or call Technical Service. Follow the protocol below to purify PCR products. The volumes given are on a per sample basis. 1. To ~50 µl PCR samples in 96-well plates, add 10 µl ChargeSwitch® Magnetic Beads.
MCDB-131, 1X Medium Cellgro 15-100-CV Barbed Polypropylene Fittings Cole Parmer Instrument Co.
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
structure. Growing Cells for Immunofluorescence We plate cells on glass coverslips (12mm circles or similar ). We pretreat coverslips typically with poly-L-lysine (PLL) if cells are loosely adherent to glass. Polyornithine is better for some neurons
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