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文献和实验Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique
with silicone grease, and the tip of a single microelectrode connected to the amplifier is then inserted within the fiber through the silicone layer. This method represents an alternative to the traditional vaseline-gap isolation
Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays
-coated beads and high vacuum silicone grease, these techniques can be adapted to a wide variety of culture conditions. They allow time-lapse study, high-resolution microscopy, and can be set up at no extra cost.
complete disruption of the tail.) 4. Quick spin tubes to get solution off inside of cap. 5. Fill inside well of microfuge tube cap with vacuum grease.(We use Dow Corning high vacuum grease and a 10cc syringe to dispense.) 6. Add 0.7 ml neutralized phenol
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