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文献和实验秀丽线虫RNA提取--C. elegans RNA prep
disposable tube. It should be brownish yellow. 6. You will need to prepare one cesium gradient for each 6 mls of homogenate. Use RNase free 16X102 mm polyallomar tubes, which fit the SW28.1 buckets (which in the Horvitz lab we spin on an SW28 rotor). Don
RNase free 16X102 mm polyallomar tubes, which fit the SW28.1 buckets (which in the Horvitz lab we spin on an SW28 rotor). Don't use " ultraclear" tubes; these crack during the spin. Measure out 11.5 mls of the cesium solution (use a disposable plastic
, MA) 50 X Tris-Acetate Electrophoresis BufferM Agarose Dye Solution (Xylene Cyanol/Bromophenol Blue) (e.g. #351-081-030, Quality Biological Inc., Gaithersburg MD) Glycerol (enzyme grade) Ethidium Bromide solution (10 mg/ml) 100 base-pair
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