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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
, clearance mounting hole Newport SN100C-F 2 High Precision Knob Adjustment Screw, 12.7mm travel, 100TPI Newport
A/B/C Random Amplification Protocol
for an additional 8 min to complete the first cycle. 7. During the second cycle (again at 10ºC) spike each sample with 1.2 µL of Reaction 4 mix. 8. When the cycling is complete add 43.8 µL dH2 O to each sample to bring their final volumes to 60 µl.
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