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文献和实验Calibration of Microarray Gene-Expression Data
Calibration of microarray measurements aims at removing systematic biases from the probe-level data to get expression estimates that linearly correlate with the transcript abundance in the studied samples. The improvement of calibration
LC-MS Data Analysis for Differential Protein Expression Detection
consisting of thousands of peak intensities for peptides represented by retention time (RT) and mass-to-charge ratio (m /z ) values. In label-free differential protein expression studies, multiple LC-MS runs are compared to identify differentially abundant
product is detected in the PCR process as a significant increase in fluorescence, and the lower the Ct value. Ct values are very reproducible in replicates because the threshold is picked to be in the exponential phase of the PCR, where there is a linear
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