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- Beckman Coulter贝克曼库尔特
- 美国
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- 2025年07月10日
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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
5, was used for transformation by vacuum infiltration. Construction of pGKB5 A new plant transformation vector was designed, that could be used for T-DNA tagging in Arabidopsis. The T-DNA region of this plasmid is flanked by fragments containing
. 4). 10) Place a sterile syringe filter into the air vent (Fig. 4). 11) Move the flow circuit into a humidified incubator at 37 °C, 5% CO2 . 12) Clamp the hard tubing into the roller pump head indicated for use with this type
. It should be noted that several commercial kits for performing microdialysis are also available. For example, use of microdialysis cassettes (Slide-A-Lyzer, Pierce or Dispo-Dialyzer, Spectrum) eliminates the need for boiling or presoaking dialysis tubing
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