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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
is usually good for 24-36 hours of use). 7. Turn on main power. 8. Turn on main service valves for steam, water and air. 9. Open air, water, and steam valves on fermentor. Check for leaks. 10. Make sure seal steam and seal steam condenser
Buffer G2,最大速漩涡10-30s,充分重悬核酸沉淀物(时间要控制得很严格)。(10)加入25μl QIAGEN Protease,50℃保育30-60min(如有必要可延长保育时间)。2)DNA的提取二、基因组步行法扩增5’及3’侧翼序列1 材料基因组DNA2.仪器、用具 离心机、PCR仪 3.试剂 (1)30μl DraⅠ(10units/μl) (2)10μl T4 DNA Ligase (6units/μl) (3)10×Ligation Buffer (4)BD Genome
A quick RNA mini-prep for Neurospora mycelial cultures
is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C
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