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文献和实验CCMB buffer to make chemical competent cell
buffer 5 Measurement of competence 6 5x Ligation Adjustment Buffer 7 References 1.Preparing glassware and media Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free
TOP10 chemically competent cells
should yield around 100 - 400 colonies Transformation efficiency is (dilution factor=15) x colony count x 105 /µgDNA We expect that the transformation efficiency should be between 5x108 and 5x109 cfu/µgDNA 5x Ligation Adjustment
the needle. Set up the scope and inector: We use a Zeiss Axiovert 10 microscope; the relevant objectives are the plan neofluor 5X and 40X. Mounted on the scope is a Narishige Model MO-202 micromanipulator, on which is mounted a needle holder hooked
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