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文献和实验A safe method of extracting DNA from Coccidioides immitis
, Academic Press, San Diego). The pellet is resuspended in 100 ul TE, and 5 ul of this is electrophoresed in agarose to check the yield - usually there is an easily visible band of high molecular weight DNA (Figure 1). We have successfully amplified the DNA
Preparation of Sonicated Human DNA
in an ice bucket. Wash the micro-tip with 70% EtOH and ddH2O before and after each use. Combine the samples and run 1 � of sonicated DNA on a 0.8% TA-agarose gel at 50V for 1 hour with BRL 1kb ladder as a marker.
DNA fingerprinting - agarose gel
, with 1 µl of a standard marker in the first and every fifth well. Marker is a mixture of 1 kb ladder (Life Technologies) and both Marker II and Marker III (Boehringer-Mannheim) in these proportions: 0.83 µl (1=B5g/µl), 3.33 µl (250 ng/µl) Marker II, 3.33 µ
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