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文献和实验RNase and DEPC Treatment: Fact or Laboratory Myth
. Figure 1. Effect of Autoclaving on RNase Activity. Various concentrations of RNase A were added to PBS and autoclaved for 25 minutes. 1 µl of each solution was mixed with 1 ng of a 5 x 104 cpm RNA probe which was 304 bases long and incubated
Replication timing by density transfer
volume of a 2x enzyme mix that contains the appropriate restriction enzyme buffer, restriction enzyme (usually 1 microliter per sample) and 0.25 microgram/ml RNase A. If the DNA prep is clean, the digest can go overnight at 37 degrees C.] Choosing
100g/200g 140/260 Amresco分装 B1008 Acryl/Bis 19:1, 30% Solution 250ml 150 进口原料自产 B1009 Acryl/Bis 29:1, 30% Solution 250ml
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