货号：VWRV1B1409 AMRESCO HOT ST

Taq酶PCR实验方法介绍

for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended

PCR基础知识[PCR basics]

is repeated over and over。 The DNA polymerase, known as 'Taq polymerase', is named after the hot-spring bacterium Thermus aquaticus from which it was originally isolated. The enzyme can withstand the high temperatures needed for DNA-strand separation

Single tube confirmation PCR protocol

until the PCR is started. Alternatively, a "hot start" can be performed by adding the Taq polymerase to the individual tubes after the PCR mixtures have been heated to 94°C. Hot start improves the PCR results but this step is labor intensive