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麦飞生物
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文献和实验monoclonal antibody were obtained from Sigma Aldrich Co. Ltd (Gillingham, Dorset, UK).2.2. Human Umbilical Vein Endothelial Cell (HUVEC) Isolation and CultureFollowing cannulation of the umbilical vein, 50ml of warm sterile phosphate buffered saline (PBS
Purification of Endotoxin Free mAbs
1) Endotoxin - you must assume that all standard laboratory glassware will be coated with loads of endotoxin. Wherever possible use sterile, specifically endotoxin-free (clinical grade) disposable plastics. If you need to use glassware or other materials
RNA Whole Mount In Situ Hybridization
.) In addition, all solutions containing Tween-20 should be filtered and kept sterile. 10X PBS (1 liter): 80 g NaCl 2 g KCl 14.4 g Na2PO4 2.4 g KH2PO4 (adjust to pH 7.4 with HCl and bring to 1 liter with water) PBT
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