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- AMRESCO
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- VWRV0136
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
/
- 英文名：
/
- 库存：
999
- 供应商：
麦飞生物
- CAS号：
124-68-5
- 规格：
4 L
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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
This web site was designed to provide detailed protocols for genetic footprinting, a technique for high-resolution mapping of the functional organization of cloned genes (Singh, Crowley and Brown (1997) PNAS 94: 1304-1309
Purification of Endotoxin Free mAbs
purity is required. Ion exchange, rather than affinity purification (eg protein A or G) is recommended, because ion exchange materials are high capacity, cheap enough to be disposable, and can easily be sanitized with 0.5M NaOH. The following method
Filtration 1. Sample: 5–10 mL pooled Wnt3A-containing fractions fromSection 2.3.2 . 2. HiLoad 26/60 Superdex 200 preparative grade. 3. Buffer: 1× PBS, 1% (w/v) CHAPS. 4. Syringe (30 mL) with 0.2-μm pore size filter. 2.3.4. Step 4: Heparin
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