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麦格生物
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文献和实验Fluorescence In Situ Hybridization
Incubate at 37ºC in humidified chamber, up-side-down, 1-2 days WAHSING Remove rubber cement Wash with 50% formamide/2xSSC at 43ºC, 10 min, 3 times, coverslip falls off in 1st wash Wash with 2xSSC at 43ºC, 10 min Wash 2xSSC/0.1% NP-40 at 43ºC
In Situ Hybridization Protocols
containing towels soaked in 50% formamide and 5xSSC. B: Washing Steps Rinse cultures in 0.2 x SSC and then wash in 0.2 x SSC at 60deg.C for 1 hour. Adjust cultures to room temperature in 0.2 x SSC for 5 minutes. Block cultures
IN SITU HYBRIDIZATION ON FROZEN SECTIONS
box with a piece of filter paper which has been saturated with Box Buffer (4xSSC, 50% formamide) on the bottom. Cover each section with 100µl of rHB2 without probe (can use 50µl if the tissue is small). Incubate at 42°C for 1-3 hours.
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