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麦格生物
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15 ml
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文献和实验【共享】Present and future applications of gold in rapid assays
no reagents other than the protein, diluent buffers, and particles. With defined raw materials, IVD manufacturers can calculate the exact amount of antibody required per particle of gold to give optimal assay performance, which results in cost savings
100μl of standard or sample to each well in triplicate. Incubate at room temperature for at least 2 hours. 2) Detection: Wash plate four times. Dilute detection antibody (biotinylated) in diluent to a concentration of 0.5μg/ml (500ng/ml
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
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