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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见瓶身
- 保质期:
/
- 英文名:
/
- 库存:
999
- 供应商:
麦格生物
- CAS号:
151-21-3
- 规格:
100 ml
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文献和实验8.8 (resolving gel) 1.0 M Tris, pH 6.8 (stacking gel) 5x SDS Running Buffer (1 L) Tris 15 g Glycine 72 g SDS 5 g Coomassie Blue Stain 10% (v/v) acetic acid 0.006% (w/v) Coomassie Blue dye 90% ddH2O Isopropanol Fixing Solution 10%
can be made by dissolving 15.1 g of Tris base and 94 g of glycine in 900 ml of deionized H2 O. Then, 50ml of a 10% (w/v) stock solution of electrophoresis-grade SDS is added, and the volume is adjusted to 1000 ml withH2 O 3. Resolving gel 4X buffer 1.4M Tris
You will need the following reagents: 5x Sample Buffer 10% w/v SDS 10 mM Dithiothreitol, or beta-mercapto-ethanol
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