货号：VWRVM111 AMRESCO SDS 10%

SDS

8.8 (resolving gel) 1.0 M Tris, pH 6.8 (stacking gel) 5x SDS Running Buffer (1 L) Tris 15 g Glycine 72 g SDS 5 g Coomassie Blue Stain 10% (v/v) acetic acid 0.006% (w/v) Coomassie Blue dye 90% ddH2O Isopropanol Fixing Solution 10%

SDS-page minigel

can be made by dissolving 15.1 g of Tris base and 94 g of glycine in 900 ml of deionized H2 O. Then, 50ml of a 10% (w/v) stock solution of electrophoresis-grade SDS is added, and the volume is adjusted to 1000 ml withH2 O 3. Resolving gel 4X buffer 1.4M Tris

SDS Gel Electrophoresis

  You will need the following reagents: 5x Sample Buffer 10% w/v SDS 10 mM Dithiothreitol, or beta-mercapto-ethanol