产品简介:
规格:50 µg
纯度:>90%,通过SDS-PAGE使用银染色进行可视化,并通过考马斯亮蓝染色进行定量测定。
内毒素水平:通过LAL方法测定每1 μg蛋白质<0.10 EU。
活性:通过功能性ELISA中的结合能力来测量。当重组人CD19 Fc嵌合体以2 µg/mL,100 µL每孔固定时,重组生物素化人CD81 Fc嵌合体的结合ED50为4-20 µg/mL。
来源:中国仓鼠卵巢细胞系,CHO衍生的人CD19蛋白
Accession#:P15391
N-端序列:Glu21
结构:二硫键连接的同型二聚体
预测分子量:57 kDa
SDS-PAGE:77-92 kDa,还原条件
形式:从0.2 µm过滤的PBS溶液中冻干。
复溶:在PBS中以500 μg/mL重新配制。
运输:产品在环境温度下运输。收到后,立即将其存放在下文建议的温度下。
储存/稳定性:避免重复冻融循环。复溶后,在无菌条件下2至8℃,1个月;-20至-70℃,3个月。
产品订购:
货号 |
产品名称 |
规格 |
9269-CD |
Recombinant Human CD19 Fc Chimera Protein, CF |
50 µg |
CD19 protein is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker (1). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (4, 5). The CD19 protein associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13, (6-9). These associations enable CD19 to amplify B cell signaling (7, 9-12) and reduce the threshold for antigen stimulation through the BCR (13). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers. The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials.
Mature human CD19 protein consists of a 272 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 22 aa transmembrane segment, and a 243 aa cytoplasmic domain (3). Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19.