R&D重组人 IL-17D蛋白

R&D重组人 IL-17D蛋白(1504-IL)

产品简介：

规格：25 µg

纯度：>97%，在还原条件下进行SDS-PAGE并通过银染进行可视化。

内毒素水平：通过鲎试剂法测定，每 1 μg 蛋白质<1.0 EU。

活性：通过功能性ELISA 中的结合能力来测量。当重组人 IL-17D 的浓度为 1 μg/mL 时，产生50% 最佳结合反应的重组人IL-17B R Fc Chimera（1207-BR）的浓度约为0.4-2 μg/mL。

来源：大肠杆菌衍生的人IL-17D 蛋白

Accession#：Q8TAD2.1

N-端序列：Ala18

结构：二硫键连接的同型二聚体

预测分子量：20 kDa（单体）

复溶：在无菌的4mM HCl中以100 μg/mL重新配制。

运输：产品在环境温度下运输。收到后，立即将其存放在下文建议的温度下。

储存/稳定性：避免重复冻融循环。复溶后，在无菌条件下2至8℃，1个月；-20至-70℃，3个月。

The Interleukin-17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a
non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL‑17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and
IL‑17 RE, have also been reported (1-4). The functions of IL-17 RC, D, and E are not known.

Human IL-17D cDNA encodes a 202 amino acid (aa) residues protein with a putative 17 aa signal peptide (5). Human and mouse IL-17D share 78% sequence identity. Among IL-17 family members, IL-17D is most closely related to IL-17B, sharing 27% aa sequence homology (5, 6). IL-17D is expressed preferentially in skeletal muscle, heart, adipose tissue, lung, pancreas, and nervous system (1, 5). Like other IL-17 family members, IL-17D modulates immune responses indirectly by stimulating the production of myeloid growth factors and chemokines including IL-6, IL-8, and GM-CSF (5). IL-17D has also been shown to suppress the proliferation of myeloid progenitors in colony formation assays. The receptor of IL-17D has not yet been identified. However, stimulation of IL-8 production by IL-17D is mediated through the activation of nuclear factor kappa-B (5). The IL-17D preparations from R&D Systems have been found to bind immobilized recombinant IL17B R/Fc in a functional ELISA.