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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Recombinant Human Angiopoietin-2 Protein
- 供应商：
北京百奥创新科技有限公司
- 规格：
25 µg
R&D重组人TREM2 His-tag蛋白,CF(623-AN)
产品简介：
规格：25 µg
纯度：>97%，通过 SDS-PAGE 进行可视化，通过银染色和考马斯®蓝染色进行定量密度测定。
内毒素水平：通过LAL方法测定每1 μg 蛋白质<0.10 EU。
活性：通过其激活转染人Tie-2 的C6大鼠神经胶质细胞中Tie-2 的能力来衡量。0.2 μg/mL 重组人血管生成素-2显着诱导人Tie-2磷酸化。
来源：小鼠骨髓瘤细胞系，NS0 衍生的人血管生成素-2 蛋白
Accession#：O15123
N-端序列：Tyr19
结构：Oligomer
预测分子量：56 kDa
SDS-PAGE：66 kDa，还原条件
形式：从0.2 µm过滤的含有海藻糖的MOPS，NaCl，CHAPS和PEG溶液中冻干。
复溶：在PBS中以100 μg/mL重新配制。
运输：产品在环境温度下运输。收到后，立即将其存放在下文建议的温度下。
储存/稳定性：避免重复冻融循环。复溶后，在无菌条件下2至8℃，1个月；-20至-70℃，3个月。
| 货号 |
产品名称 |
规格 |
| 623-AN |
Recombinant Human Angiopoietin-2 Protein |
25 µg |
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled‑coil domain (aa 166-248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275-495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65-66 kDa in size. Under non‑reducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250-300 kDa tetramers and pentamers (3-6). Alternate splicing generates a short isoform that lacks 52 amino acids (aa) preceding the coiled‑coil domain (4). Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1, 3, 7). Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang-1 is a potent Tie‑2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang-2 becomes as a Tie-2 agonist (3, 8‑11). The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2 (4). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (12, 13). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (8, 13, 14). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development (7, 15). It mediates the up‑regulation of ICAM‑1 and VCAM‑1 on EC, which facilitates the adhesion of leukocytes during inflammation (16). Ang-2 is up‑regulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (17-20). Its direct interaction with Integrins promotes tumor cell invasion (21, 22). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (20).
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文献和实验罗氏蛋白亲和纯化树脂：cOmplete His-Tag Purificatio
cOmplete His-Tag Purification Resin 灵活纯化蛋白、保证效能 选择靶蛋白适宜的缓冲条件 cOmplete His-Tag Purification Resin是一种新型的高效能IMAC基质，用于裂解液中His标签蛋白的一步法纯化，操作方便。采用罗氏特殊的镍螯合化学
材料 琼脂糖凝胶介质镍亲和柱 紫外检测仪 蛋白电泳装置及夹子、玻璃板、灌胶支架、缓冲液槽等附件 5 ml 注射器 0.45 μm 滤器 超声破碎仪 试剂 1. 结合缓冲液：20 mM Tris-HCl，150 mM NaCl，10 mM 咪唑，pH=8.0 2. 洗脱缓冲液：20 mM Tris-HCl，150 mM NaCl，不同梯度浓度的咪唑，pH=8.0 3. 20%乙醇 4. 三蒸水 5. 透析液：20 mM Tris
质，小分子或是金属。当融合有亲和标签的蛋白通过标签-配体作用结合再层析基质上时，通过洗杂步骤，即可去除掉其他的细胞成分。 为了洗脱所需要的蛋白质，通过改变缓冲液的条件，如pH，或通过竞争亲和标签和配体连接的方法来进行。 但怎样的纯化是较为成功的呢？仅达到所需的质量量级，如毫克级（或克级）即可满足吗？ 这些并不简单。质在蛋白纯化中同样尤为重要，比如，就蛋白的生物活性和纯度而言，通常会有所要求。 以下我们来讨论并且比较2种技术： His-tag系统和Strep-tag®系统，都使用了亲和层析法来纯化
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