R&D重组小鼠 IL-17D 蛋白

R&D重组小鼠 IL-17D 蛋白(2274-ML)

产品简介：

规格：25 µg

纯度：>95%，通过SDS-PAGE在还原条件下进行，并通过银染剂可视化。

内毒素水平：通过鲎试剂法测定每1 μg蛋白质<0.01 EU。

活性：通过其在功能性ELISA 中结合rmIL-17B R/Fc嵌合体的能力来测量。

来源：大肠杆菌来源的小鼠IL-17D蛋白

Accession#：Q8K4C4

N-端序列：Ala25

结构：二硫键连接的同型二聚体

预测分子量：20 kDa（单体）

形式：从0.2 µm过滤的yi腈和TFA溶液中冻干。

复溶：在4 mM HCl中以100 μg/mL重新配制。

运输：产品在环境温度下运输。收到后，立即将其存放在下文建议的温度下。

储存/稳定性：避免重复冻融循环。复溶后，在无菌条件下2至8℃，1个月；-20至-70℃，3个月。

货号	产品名称	规格
2274-ML	Recombinant Mouse IL-17D Protein	25 µg

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2, 6). With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2, 6). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1 - 6). Mouse IL-17D is synthesized as a 205 amino acid (aa) precursor that contains a putative 24 aa signal peptide and a 181 aa mature segment. The mature region contains two potential N-linked glycosylation sites and eight cysteines, four of which are involved in the formation of a modified cysteine-knot motif (5). The molecule is reported to exist as a 53 kDa disulfide-linked homodimer (2, 5). Given that its predicted homodimeric molecular weight is 40 kDa, the molecule is presumably glycosylated. In the mature region, mouse IL-17D is 88% aa identical to human IL-17D. There is less than 30% aa identity between mouse IL-17D and other members of the mouse IL-17 family. IL-17D is expressed in skeletal muscle, adipose tissue, fetal liver, and heart, plus resting CD4⁺ T cells and CD19⁺ B cells (1). R&D Systems has shown IL-17D binding to a mouse IL-17 R/Fc construct in a functional ELISA. IL-17D is known to induce the production of IL-8, IL-6 and GM-CSF (5).