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- 保存条件:
/
- 保质期:
1-3年
- 英文名:
明胶 来源于猪皮肤
- 库存:
999
- 供应商:
麦飞生物
- CAS号:
9000-70-8
- 规格:
100g
说明
应用
This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.
组分
明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。
注意
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
制备说明
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 80-120.
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文献和实验Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy.
Rui Han et al.
Nanoscale, 14(33), 12060-12068 (2022-08-11)
新突破!科学家用猪皮中的胶原蛋白造出了眼角膜,14 位失明患者重见光明
8 月 11 日,瑞典林雪平大学生物医学与临床学系眼科 Neil Lagali 教授团队带来了一个或可令视障人士重见光明的新发现。该团队在 Nature Biotechnology 杂志发表研究论文 Bioengineered corneal tissue for minimally invasive vision restoration in advanced keratoconus in two clinical cohorts,报道了一种类似于人类眼角膜的新型生物工程角膜,这种新型角膜由来自猪皮肤
「头等大事」,追本溯源!Nature 重磅报道毛囊干细胞起源
形成。邻近皮肤乳头的细胞保留在上皮细胞和皮肤乳头之间,或脱离基底膜分化。这些结果表明,毛囊形态发生是每一个纵向排列的上皮细胞间隔的扩大,而不是主要的毛发生殖细胞的继续增加。图片来源:Nature为了探究毛囊上皮细胞起源和早期世系动力学,研究人员利用小鼠模型获得了 hair placode stage 的实时图像数据。随后进行回顾性细胞追踪,发现位于皮肤乳头附近的上皮细胞最初位于基板基底层的中心。下层区域的细胞来源于基板的中心区域外,上部的细胞(putative prospective bulge
〔 1〕是生于哺乳类皮肤中的丝状角质物,在体表的大部分都生有毛,一般形成毛被。毛是由表皮内陷后形成的毛囊基部向外生长而成的。包在毛囊中的部分称毛根,露出体外的部分称毛干。毛的前端细而尖,称毛尖。由表皮内陷形成的毛囊为表皮性毛囊( epidermic hair follicle),包在其外面的由结缔组织形成的囊称真皮性毛囊( dermic hair folli-cle)。在表皮性毛囊与真皮往毛囊之间,有相当于基底膜的称为玻璃膜( glassy
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