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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
986
- 靶点:
β2-microglobulin
- 级别:
β2-microglobulin Antibody
- 目录编号:
β2-microglobulin Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
β2-microglobulin Antibody
- 抗体英文名:
Fire™ 750 anti-human β2-microglobulin Antibody-APC
- 抗体名:
β2-microglobulin Antibody
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
β2-microglobulin Antibody
- 亚型:
β2-microglobulin Antibody
- 形态:
β2-microglobulin Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
β2-microglobulin Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥3000.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6500.0 |
Description :β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membraneanchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavychains of MHC class I molecules to form HLA class I antigen complex. In association with HLAclass I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2Mplays an essential role both in governing MHC class I molecules stability and in promotingantigen binding and presenting the antigen to CD3/TCR complex of CD8 T cells.
Verified Reactivity :Human
Reported Reactivity :African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Purified human β2-microglobulin
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
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文献和实验1. Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed
2. Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed
3. Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
常用抗体标记荧光染料的选择 随着免疫荧光技术的不断发展,荧光染料及其标记的抗体偶联物也被广泛的应用于生物学实验中。目前,市场上抗体及蛋白标记的荧光染料主要有CFTM系列(BIOTIUM, USA); Alexa Fluor®系列 (Life technology, USA); DyLight系列;Cy系列; IR Dye系列等等。使用最多的为AlexaFluor®系列和CFTM系列。CFTM系列染料的核心对比AlexaFluor®系列具有以下几点优势:1. 新型罗丹明核心罗丹明染料以优异
以花青或罗丹明染料为核心结构,该核心结构的特殊化学修饰限制了染料的分子内位移,从而获得染料的高量子产率和更好的水溶性。因此,近红外CF染料比其他近红外染料荧光强度更高,光稳定性好。最重要的是,在免疫印迹实验上应用近红外CF染料标记蛋白质样品相对于其他商业用近红外染料制备的抗体偶联物,最大限度的提高了信噪比。 3、优异的标记效率 生物偶联后的活性染料一般很容易水解,会在运输,操作及储存中带来诸多不便,最终致染料结合效率低下。像Alexa Fluor®dyes,DyLight™dyes











