Description :CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocytecommon antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasmamembrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signalingmolecule that regulates a variety of cellular processes including cell growth, differentiation, cellcycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigenreceptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, andother Src family kinases. CD45 non-covalently associates with lymphocyte phosphataseassociated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bindgalectin-1 and to be associated with several other cell surface antigens including CD1, CD2,CD3, and CD4.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human PBMC
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.