Description :CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some folliculardendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated onactivated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought toform Ca conductive ion channels in the plasma membrane of B cells. The CD20 molecule isinvolved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). Itexists in a complex with MHC class I and II, CD53, CD81, and CD82.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.