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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
986
- 靶点:
CD20
- 级别:
CD20 Antibody
- 目录编号:
CD20 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD20 Antibody
- 抗体英文名:
Cyanine7 anti-human CD20 Antibody-APC
- 抗体名:
CD20 Antibody
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
人、猴
- 免疫原:
CD20 Antibody
- 亚型:
CD20 Antibody
- 形态:
CD20 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD20 Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2600.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥5300.0 |
Description :CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some folliculardendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated onactivated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought toform Ca conductive ion channels in the plasma membrane of B cells. The CD20 molecule isinvolved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). Itexists in a complex with MHC class I and II, CD53, CD81, and CD82.
Verified Reactivity :Human, Cynomolgus, Rhesus
Reported Reactivity :Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human tonsillar B cells
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations:
1. Lewis M, et al. 2015. Am J Hum Genet. 96:221. PubMed
2. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
3. Ratliff M, et al. 2015. J Immunol. 194:940. PubMed
4. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
5. Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
6. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
7. Fraussen J, et al. 2019. J Immunol. 203:1650. PubMed
8. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
9. Whittle J, et al. 2014. J Virol. 88:4047. PubMed
10. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
11. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
12. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
CD20抗体Fab’片段,并在大肠杆菌中高效可溶性分泌表达[ 8 ] ,本研究的目的在于研究嵌合抗CD20Fab’片段的抗肿瘤活性及其作用机制。一、材料和方法1、材料MTT购自Sigma公司;Annexin-V为宝灵曼公司试剂盒;TRIZOL RNA提取试剂盒为美国Gibco公司产品;兔抗人bcl-2单克隆抗体和辣根过氧化物酶标记的羊抗兔IgG为北京中山生物技术有限公司产品,嵌合抗CD20Fab’片段为我室表达并纯化。β-actin引物(上游:5-TCATGTTTGAGACCTTCAA-3,下游
69、CD38 等活化指标,及 IFN-γ、IL-4、IL17A 等细胞因子,则需要设置同型对照,实现阴阳性细胞群的良好区分。 CD4 CD25 (2)在某些特殊情况下,如单核细胞被 PE/Cyanine 7、APC/Cyanine 7 等串联染料标记的抗体染色时,使用同型对照抗体是十分有必要的。单核细胞一般倾向于与 Cyanine7 等染料非特异性结合,使用同型对照抗体有助于获得理想结果。 (3)不清楚抗原表达情况的时候,一定要使用同型对照来界定阴性细胞群。 (4)胞内染色时
临床验证。抗人CD20(Rituximab)是第1个为FDA批准可用于临床癌症治疗的单抗。Rituximab与细胞表面的CD20分子结合后,可通过诱导ADCC和CDC清除高表达CD20分子的恶性B细胞,从而被认为在低分化的B细胞淋巴瘤和非霍奇金淋巴瘤的治疗中起重要作用。 近年来,另一个备受人们关注的治疗性单抗是抗Her-2的抗体。Her-2是表皮生长因子受体(EGFR)成员,参与乳腺癌的发生,且与乳腺癌患者的预后有关。相应单抗与Her-2结合可阻止Her-2与相应的生长因子的结合,抑制乳腺










