- ￥2500
- Vacara
- 10110
- 瑞典
- 2025年07月14日
- Functional assays， IHC
- 小鼠
- Mouse, rat, human, bovine, chicken
8 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
北京博蕾德生物科技有限公司
- 库存：
1
- 靶点：
col2A1
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 抗体英文名：
Mouse Anti col2A1 Antibody (M2139)
- 抗体名：
Mouse Anti col2A1 Antibody (M2139)
- 宿主：
小鼠
- 适应物种：
Mouse, rat, human, bovine, chicken
- 免疫原：
Mouse collagen II J1 epitope (triple helical position 551-564) with sequence MPGERRGAAGIAGPK
- 亚型：
Mouse IgG2b, κ
- 形态：
液体
- 应用范围：
Functional assays， IHC
- 保存条件：
-20℃
- 浓度：
1 mg/ml
- 规格：
100µg/0.1mL
Description
Affinity purified mouse monoclonal anti collagen II (COL2A1) antibody clone M2139 in PBS, sterile filtered (20 µm). The antibody recognizes the J1 epitope on triple helical collagen II1. The J1 epitope on mouse collagen consist of residues 551–564 with sequence MPGERRGAAGIAGPK1,4. The antibody M2139 has been used in applications including immunohistochemistry4, Western blot2, immunoprecipitation3, and in vivo4 and in vitro5 functional assays.
Target with alternative names: Collagen alpha-1(II) chain, COL2A1, type II collagen, CII; UniProt: P28481.
Immunogen: Mouse collagen II J1 epitope (triple helical position 551-564) with sequence MPGERRGAAGIAGPK1,4.
Species reactivity: Mouse, rat, human, bovine, chicken
Isotype: Mouse IgG2b, κ
Specificity: The antibody M2139 recognizes the conformational J1 epitope on the triple-helical structure of the native CII molecule.
Concentration: 1 mg/ml
Supplied in: PBS
Storage: Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at 20°C long term. Avoid repeated freeze/thaw cycles.
Recommended dilution: It is recommended the user determines the optimal dilution for their application. The typical starting working dilutions are: Functional assays (cell culture) 1:20–1:50, IHC 1:100.
Affinity purified mouse monoclonal anti collagen II (COL2A1) antibody clone M2139 in PBS, sterile filtered (20 µm). The antibody recognizes the J1 epitope on triple helical collagen II1. The J1 epitope on mouse collagen consist of residues 551–564 with sequence MPGERRGAAGIAGPK1,4. The antibody M2139 has been used in applications including immunohistochemistry4, Western blot2, immunoprecipitation3, and in vivo4 and in vitro5 functional assays.
Target with alternative names: Collagen alpha-1(II) chain, COL2A1, type II collagen, CII; UniProt: P28481.
Immunogen: Mouse collagen II J1 epitope (triple helical position 551-564) with sequence MPGERRGAAGIAGPK1,4.
Species reactivity: Mouse, rat, human, bovine, chicken
Isotype: Mouse IgG2b, κ
Specificity: The antibody M2139 recognizes the conformational J1 epitope on the triple-helical structure of the native CII molecule.
Concentration: 1 mg/ml
Supplied in: PBS
Storage: Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at 20°C long term. Avoid repeated freeze/thaw cycles.
Recommended dilution: It is recommended the user determines the optimal dilution for their application. The typical starting working dilutions are: Functional assays (cell culture) 1:20–1:50, IHC 1:100.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
1. Mo JA, Holmdahl R. The B cell response to autologous type II collagen: biased V gene repertoire with V gene sharing and epitope shift. J Immunol. 1996 Sep 15;157(6):2440-8. PMID: 8805643.
2. Tong D, Lönnblom E, Yau ACY, Nandakumar KS, Liang B, Ge C, Viljanen J, Li L, Bãlan M, Klareskog L, Chagin AS, Gjertsson I, Kihlberg J, Zhao M, Holmdahl R. A Shared Epitope of Collagen Type XI and Type II Is Recognized by Pathogenic Antibodies in Mice and Humans with Arthritis. Front Immunol. 2018 Apr 12;9:451. PMID: 29706949.
3. Uysal H, Bockermann R, Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H, Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449-62. PMID: 19204106.
4. Li Y, Tong D, Liang P, Lönnblom E, Viljanen J, Xu B, Nandakumar KS, Holmdahl R. Cartilage-binding antibodies initiate joint inflammation and promote chronic erosive arthritis. Arthritis Res Ther. 2020 May 24;22(1):120. PMID: 32448385.
5. Amirahmadi SF, Whittingham S, Crombie DE, Nandakumar KS, Holmdahl R, Mackay IR, van Damme MP, Rowley MJ. Arthritogenic anti-type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells. Arthritis Rheum. 2005 Jun;52(6):1897-906. PMID: 15934095.
2. Tong D, Lönnblom E, Yau ACY, Nandakumar KS, Liang B, Ge C, Viljanen J, Li L, Bãlan M, Klareskog L, Chagin AS, Gjertsson I, Kihlberg J, Zhao M, Holmdahl R. A Shared Epitope of Collagen Type XI and Type II Is Recognized by Pathogenic Antibodies in Mice and Humans with Arthritis. Front Immunol. 2018 Apr 12;9:451. PMID: 29706949.
3. Uysal H, Bockermann R, Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H, Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449-62. PMID: 19204106.
4. Li Y, Tong D, Liang P, Lönnblom E, Viljanen J, Xu B, Nandakumar KS, Holmdahl R. Cartilage-binding antibodies initiate joint inflammation and promote chronic erosive arthritis. Arthritis Res Ther. 2020 May 24;22(1):120. PMID: 32448385.
5. Amirahmadi SF, Whittingham S, Crombie DE, Nandakumar KS, Holmdahl R, Mackay IR, van Damme MP, Rowley MJ. Arthritogenic anti-type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells. Arthritis Rheum. 2005 Jun;52(6):1897-906. PMID: 15934095.
文献支持
Vacara cat#10110 Mouse Anti col2A1 Antibody (M2139)
￥2500
