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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存
- 保质期:
12个月
- 英文名:
Recombinant Tubulin Epsilon (TUBe)
- 库存:
100
- 供应商:
科淼生物
- 规格:
50ug/50ug
| 规格: | 50ug | 产品价格: | ¥2180.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥3980.0 |
产品英文名称:Recombinant Tubulin Epsilon (TUBe)
别名:TUBE1
种属:Homo sapiens (Human)
蛋白序列:Val225~Ser472
标签:N-terminal His and GST Tag
Uniprot:Q9UJT0
表达系统:Prokaryotic expression
来源:E.coli
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文献和实验tubulin aliquots from the freezer to the 37¡C bath/benchtop). When the tubulin is nearly thawed (i.e. when there is a very small amount of ice left in the tube) transfer the tube to an ice bucket; mix it by gentle flicking ~20s later and store on ice. Use
from each tube using a plastic scraper and making sure that all tubes get rinsed once or twice after the majority of the large gelatinous pellet has been removed. To homogenize the chunky pellet resuspension, we use a Yamato "pour-through" continuous flow
Affinity Column Preparation 亲和层析柱制备
1.Prepare 1 ml of packed beads by washing them 4 times in 10 ml of d-H2O in a 15 ml tube.The beads are Act.Ultrogel 22 AcA from IBF,and stored in the coldroom.The protein binding site is via glutaraldehyde groups.2 mls of beads from the vial
技术资料







