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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human peripheral blood lymphocytes from a patient with Sézary's syndrome and infant thymocytes
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
Flow
- 抗体英文名:
CD3 Antibody [UCHT1] (PE-Cy5.5)
- 抗体名:
CD3 Antibody [UCHT1] (PE-Cy5.5)
- 规格:
100tests
CD3 抗体 [UCHT1] (PE-Cy5.5),CD3 Antibody [UCHT1] (PE-Cy5.5),CD3 抗体 [UCHT1] (PE-Cy5.5),CD3 Antibody [UCHT1] (PE-Cy5.5)
产品名称:CD3 抗体 [UCHT1] (PE-Cy5.5)-CD3 Antibody [UCHT1] (PE-Cy5.5)
产品货号:PSI-99-420-100tests
产品规格:100tests
背景资料:CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa epsilon chain of the CD3/TCR complex.
保存建议:CD3 抗体 [UCHT1] (PE-Cy5.5)-CD3 Antibody [UCHT1] (PE-Cy5.5)Store vial at 2-8℃
应用类型:Flow
点击:CD3 抗体 [UCHT1] (PE-Cy5.5)-CD3 Antibody [UCHT1] (PE-Cy5.5)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验- 3) 及 CD45 -PE (HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞以 DAPI 染色先行排除,左右分别为分选前后的细胞群分布。 总结: 几次实验的结果,使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选,平均得率为 84.5%,纯度大于 90%,细胞存活率高于 95%,且目标细胞不含标记,贴近天然,使下游实验更精确。
: 由此步骤开始,流出的溶液包含目标细胞,请使用新的试剂管收集!首先,加入 1 ml 的 biotin 洗脱液,孵育 2 min。其次,加入 10 ml biotin 洗脱液,洗脱目标细胞。d) 再加入 10 ml biotin 洗脱液,洗下剩余的目标细胞。最后两步骤所收集的液体含分选出的目标细胞。(4) 结果分析以下流式图为从 7.5 ml 全血中,选出 CD3+ T 细胞的数据。选后细胞以 CD3-APC(OKT-3) 及 CD45-PE(HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞
: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
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