- ￥22
- ProSci
- 美国
- PSI-98-743-0.1mg
- 2025年07月11日
- WB, WB-NR, IHC-P, IHC-Fr, Flow, IP
- 大鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
VH81X Tg B cells and T220 stromal cell line
- 保存条件：
Store vial at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
大鼠
- 应用范围：
WB, WB-NR, IHC-P, IHC-Fr, Flow, IP
- 抗体英文名：
CD16/32 Antibody [93] (APC)
- 抗体名：
CD16/32 Antibody [93] (APC)
- 规格：
0.1mg
CD16/32 抗体 [93] (APC)，CD16/32 Antibody [93] (APC)，CD16/32 抗体 [93] (APC),CD16/32 Antibody [93] (APC)
产品名称：CD16/32 抗体 [93] (APC)-CD16/32 Antibody [93] (APC)
产品货号：PSI-98-743-0.1mg
产品规格：0.1mg
背景资料：The lymphocyte Fcgamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fcgamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying FcgammaR+ cells, monoclonal antibodies to the FcgammaII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on FcgammaR for parasitic invasion. The monoclonal antibody 93 recognizes a conformational epitope formed by FcgammaII and FcgammaIII receptors.
保存建议：CD16/32 抗体 [93] (APC)-CD16/32 Antibody [93] (APC)Store vial at 2-8℃
应用类型：WB, WB-NR, IHC-P, IHC-Fr, Flow, IP
点击：CD16/32 抗体 [93] (APC)-CD16/32 Antibody [93] (APC)查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750（每次测试 5μL），以及 CD16-FITC（每次测试 20 μL），并在冰上孵育 30 分钟。10. 在 4℃ 条件下，300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集：1. 创建新补偿值。2. 如适用
#01 什么是同型对照？ 同型对照（Isotype Control），是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度，但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体与抗原的非特异性结合造成的背景染色。 #02 为什么要做同型对照？ 流式抗体与靶标的结合，是通过抗体的 Fab 端与靶标 Marker 进行特异性结合而完成的。白细胞（除T细胞外）表面大多表达 CD16、CD32、CD64 等 Fc 受体，这些受体可与各种抗体的 Fc 端非特异性结合，出现非特异
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
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