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- ProSci
- 美国
- PSI-79-243-0.1mg
- 2025年07月12日
- WB, IHC
- 兔
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
eIF4G (phospho-Ser1232) antibody was raised against a peptide sequence around phosphorylation site of serine 1232 (P-V-S (p) -P-L) derived from Human eIF4G.
- 保存条件：
Store antibody at -20℃ for up to one year
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
兔
- 应用范围：
WB, IHC
- 抗体英文名：
eIF4G (phospho Ser1232) Antibody
- 抗体名：
eIF4G (phospho Ser1232) Antibody
- 规格：
0.1mg
eIF4G (phospho Ser1232) 抗体，eIF4G (phospho Ser1232) Antibody，eIF4G (phospho Ser1232) 抗体,eIF4G (phospho Ser1232) Antibody
产品名称：eIF4G (phospho Ser1232) 抗体-eIF4G (phospho Ser1232) Antibody
产品货号：PSI-79-243-0.1mg
产品规格：0.1mg
背景资料：eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
保存建议：eIF4G (phospho Ser1232) 抗体-eIF4G (phospho Ser1232) AntibodyStore antibody at -20℃ for up to one year
应用类型：WB, IHC
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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