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- 文献和实验
- 技术资料
- 免疫原:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1a (Human MIP-1 alpha ). Human MIP-1a specific antibody was purified by affinity chromatography and then biotinylated.
- 保存条件:
MIP-1a antibody is stable for at least 2 years from date of receipt at -20℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
E, WB
- 抗体英文名:
MIP-1a Antibody (biotin)
- 抗体名:
MIP-1a Antibody (biotin)
- 规格:
0.05mg
MIP-1a 抗体 (biotin),MIP-1a Antibody (biotin),MIP-1a 抗体 (biotin),MIP-1a Antibody (biotin)
产品名称:MIP-1a 抗体 (biotin)-MIP-1a Antibody (biotin)
产品货号:PSI-38-222-0.05mg
产品规格:0.05mg
背景资料:Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
保存建议:MIP-1a 抗体 (biotin)-MIP-1a Antibody (biotin)MIP-1a antibody is stable for at least 2 years from date of receipt at -20℃
应用类型:E, WB
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
Identification of Deacetylase Substrates with the Biotin Switch Approach
The identification of lysine-acetylated proteins and deacetylase substrates has primarily relied on protein immune-affinity techniques with antibodies that recognize acetylated lysine residues (Kac antibodies). While these antibody-based
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