- ￥22
- ProSci
- 美国
- PSI-38-216-0.05mg
- 2025年07月13日
- E, WB
- 兔
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1). Human TIMP-1 specific antibody was purified by affinity chromatography and then biotinylated.
- 保存条件：
TIMP-1 antibody is stable for at least 2 years from date of receipt at -20℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
兔
- 应用范围：
E, WB
- 抗体英文名：
TIMP-1 Antibody (biotin)
- 抗体名：
TIMP-1 Antibody (biotin)
- 规格：
0.05mg
TIMP-1 抗体 (biotin)，TIMP-1 Antibody (biotin)，TIMP-1 抗体 (biotin),TIMP-1 Antibody (biotin)
产品名称：TIMP-1 抗体 (biotin)-TIMP-1 Antibody (biotin)
产品货号：PSI-38-216-0.05mg
产品规格：0.05mg
背景资料：Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/mL) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
保存建议：TIMP-1 抗体 (biotin)-TIMP-1 Antibody (biotin)TIMP-1 antibody is stable for at least 2 years from date of receipt at -20℃
应用类型： E, WB
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- 作者
- 内容
- 询问日期
文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
Identification of Deacetylase Substrates with the Biotin Switch Approach
The identification of lysine-acetylated proteins and deacetylase substrates has primarily relied on protein immune-affinity techniques with antibodies that recognize acetylated lysine residues (Kac antibodies). While these antibody-based
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