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询价![Lambda Light Chain 抗体 [HP6054]-Lambda Light Chain Antibody [HP6054]](https://img1.dxycdn.com/p/s14/2023/1218/191/8638588858174956371.jpg!wh200)
Lambda Light Chain 抗体 [HP6054]-Lambda Light Chain Antibody [HP6054]
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
Flow, IF, WB, ICC, IHC
- 抗体英文名:
Golgi Antibody [SPM581]
- 抗体名:
Golgi Antibody [SPM581]
- 规格:
100ug
Golgi 抗体 [SPM581],Golgi Antibody [SPM581],Golgi 抗体 [SPM581],Golgi Antibody [SPM581]
产品名称:Golgi 抗体 [SPM581]-Golgi Antibody [SPM581]
产品货号:PSI-34-195-100ug
产品规格:100ug
背景资料:This mAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.
保存建议:Golgi 抗体 [SPM581]-Golgi Antibody [SPM581]Aliquot and Store at 2-8℃
应用类型:Flow, IF, WB, ICC, IHC
点击:Golgi 抗体 [SPM581]-Golgi Antibody [SPM581]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验1.1920 1.4056 43 1.498 1.1972 1.4076 44 1.539 1.2025 1.4096 45 1.581
孵育(Primary antibody incubation): 参考一抗的说明书,按照适当比例用稀释一抗。 吸尽封闭液,立即加入稀释好的一抗,室温或4℃在侧摆摇床上缓慢摇动孵育一小时。如果一抗孵育一小时效果不佳,可以4℃缓慢摇动孵育过夜。或更据抗体的说明选择适当的孵育温度和时间。 回收一抗。加入Western洗涤液,在侧摆摇床上缓慢摇动洗涤5-10分钟。吸尽洗涤液后,再加入洗涤液洗涤5-10分钟。共洗涤3次。如果结果背景较高可以适当延长洗涤时间并增加洗涤次数。 注
-1), whereas markers of intracellular organelles (for example, the Golgi protein -COP), other tissue cells (for example, E-cadherin for epithelium, fibroblast surface protein) and blood (for example, glycophorin A, CD4 and CD11) were greater than 20-fold depleted
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