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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Progesterone- 11a-hemisuccinate conjugated to bovine serum albumin (Pr11a-HMS-BSA) was used as the immunogen for the Progesterone antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
WB, E, RIA
- 抗体英文名:
Progesterone Antibody [6-5E-10B]
- 抗体名:
Progesterone Antibody [6-5E-10B]
- 规格:
100ug
Progesterone 抗体 [6-5E-10B],Progesterone Antibody [6-5E-10B],Progesterone 抗体 [6-5E-10B],Progesterone Antibody [6-5E-10B]
产品名称:Progesterone 抗体 [6-5E-10B]-Progesterone Antibody [6-5E-10B]
产品货号:PSI-33-538-100ug
产品规格:100ug
背景资料:This mAb is specific for progesterone. It exhibits minimal cross reactivity with related compounds in ELISA. It reacts with Progesterone-11a-HMS-BSA: 100%; 5-beta-Pregnane-3,20-dione: 48%; 5-alpha-Pregnane-3,20-dione: 26.4%; 17-alpha-Hydroxyprogesterone: 2.5% and 20-alpha-Hydroxyprogesterone: 0.04%. Progesterone is a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland. Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Thus, progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Luteinized theca cells of normal ovary secrete progesterone. The determination of progesterone concentrations in the body fluids is of great value for endocrinological investigations in women. This mAb may prove useful in identification of ovarian tumors.
保存建议:Progesterone 抗体 [6-5E-10B]-Progesterone Antibody [6-5E-10B]Aliquot and Store at 2-8℃
应用类型:WB, E, RIA
点击:Progesterone 抗体 [6-5E-10B]-Progesterone Antibody [6-5E-10B]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验ul/孔量加入酶标板孔中,置4℃过夜或37℃吸附2小时。 c. 弃去孔内的液体,同时用洗涤液洗3次,每次3-5分钟,拍干。 d. 每孔加200ul封闭液4℃过夜或37℃封闭2小时;该步骤对于一些抗原,可省略。 e. 洗涤液洗3次;此时包被板可-20℃或4℃保存备用。 f. 每孔加50-100ul待检杂交瘤细胞培养上清,同时设立阳性、阴性对照和空白对照;37℃孵育1-2小时;洗涤,拍干。 g. 加酶标第二抗体,每孔50-100ul,37℃
人白介素-3冻干粉 标准品 1,700 IU /ampoule 人白介素-4冻干粉 标准品 1,000 IU /ampoule 人白介素-5冻干粉 标准品 5,000 IU /ampoule 人白介素-6冻干粉 标准品 100,000 IU /ampoule. 人白介素-7冻干粉 标准品 100,000 units /ampoule 人白介素-8冻干粉 标准品 1,000 IU /ampoule. 人白介素-9冻干粉 标准品 1,000 IU /ampoule. Islet细胞抗体冻干
聚丙烯酰胺凝胶电泳法(PAGE)分离血清蛋白质(核黄素-TEMED聚合系统)(图)
保存。可用N-二甲基氨基丙腈或三乙醇胺代替,但效果较差。 ④三羟甲基氨基甲烷(简称Tris)。 ⑤核黄素:避光保存。光聚合催化剂。 ⑥7%醋酸溶液(V/V)。 ⑦0.5%氨基黑10B(C22H13O12N6S3Na3)溶液:用7%醋酸溶液配制。 ⑧0.01%溴酚蓝溶液。 ⑨蔗糖。 ⑩PH8.4硼酸缓冲液(0.2mol/L硼酸盐)。 四、操作步骤: 1.贮备液的配制:按表15-2配制A、B、C、D、E、F各贮备液: 上述各贮备液都需冷藏(4℃)。尤其是C、D两贮液
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