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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8/18 antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
Flow, IF, WB, IHC-P
- 抗体英文名:
Cytokeratin 8/18 Antibody [KRT8.18/1346]
- 抗体名:
Cytokeratin 8/18 Antibody [KRT8.18/1346]
- 规格:
100ug
Cytokeratin 8/18 抗体 [KRT8.18/1346],Cytokeratin 8/18 Antibody [KRT8.18/1346],Cytokeratin 8/18 抗体 [KRT8.18/1346],Cytokeratin 8/18 Antibody [KRT8.18/1346]
产品名称:Cytokeratin 8/18 抗体 [KRT8.18/1346]-Cytokeratin 8/18 Antibody [KRT8.18/1346]
产品货号:PSI-33-683-100ug
产品规格:100ug
背景资料:Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
保存建议:Cytokeratin 8/18 抗体 [KRT8.18/1346]-Cytokeratin 8/18 Antibody [KRT8.18/1346]Aliquot and Store at 2-8℃
应用类型:Flow, IF, WB, IHC-P
点击:Cytokeratin 8/18 抗体 [KRT8.18/1346]-Cytokeratin 8/18 Antibody [KRT8.18/1346]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验5. Electorophoresis with 100-130 V in 0.25X TBE buffer for 3 hours. You may use 18 cm-height gel system and stop running after dye front comes to 12 cm point from the top. When a gel was run with 130 V, the current was less than 20 mA. You could run a gel
CD11a/CD11b/CD11c 分子 CDlla 常用单克隆抗体或代号:MHM24, 2F12; CRIS―3(LFA―lα链,整合素α1) 主要表达细胞:Leu[A5] 分子质量(kDa)和结构:spl80(整合素α) 功 能:与1CAM-l(CD54)、ICAM-2(CDl02)、ICAM-3(C1350)结合,介导细胞黏附;与JAMl结合,参与白细胞穿过内皮细胞
静置,或 37℃ 1 小时;12)II抗中可加入 0.05% 的 tween-20。13)PBS 洗 3 次各 5 分钟;14)DAB 显色 5~10 分钟,在显微镜下掌握染色程度;15)PBS或自来水冲洗 10 分钟;16)苏木精复染 2 分钟,盐酸酒精分化;17)自来水冲洗 10~15 分钟;18)脱水、透明、封片、镜检。 二、SABC法 1)脱蜡、水化。2)PBS 洗两次各 5 分钟。3)用蒸馏水或PBS配置新鲜的3%H2O2,室温封闭5~10分钟,蒸馏水洗3次。4)抗原修复。5)PBS洗5分钟
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