Sigma货号539480-100mg蛋白酶K(别名:肽链内

Sigma-Millipore-Calbiochem货号539480-100mg蛋白酶K(别名:肽链内切酶)13611631389上海睿安生物

说明

一般描述

丝氨酸蛋白酶，对多种变性和天然的高分子量蛋白质具有很强的蛋白水解活性。它可使内源性核酸酶迅速蛋白水解失活，因此可用于分离mRNA和高分子量DNA。优先裂解N-取代疏水性、脂肪族和芳香族氨基酸羰基旁的键。可被DFP、Hg²⁺和PMSF抑制。不会被金属螯合剂、巯基试剂、TLCK或TPCK灭活。

应用

在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

生化/生理作用

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp³⁹-His⁶⁹-Ser²²⁴)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

警告

毒性:刺激性(B)

单位定义:一个mAnson单位定义为在37°C、pH 7.5条件下每分钟释放1.0µM Folin阳性氨基酸(以酪氨酸计)的酶的量。

外形:由乙酸钙冻干

重悬:复溶后，储存在冰箱中(4°C)。用50mM Tris-HCl、2 mM乙酸钙制备的pH 8.0的储备溶液在4°C下可稳定保存1年。

其他说明

Watazu, Y., et al. 1993.J. Lab. Clin. Anal.7, 81.
Lebherz, H.G., et al. 1986.生物化学。J.233, 51.
Ebeling, W., et al. 1974.Eur. J. Biochem.47, 91.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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