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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Cyclin O antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Cyclin O.<br>The immunogen is located within the first 50 amino acids of Cyclin O.
- 保存条件:
stored at 4℃ for three months and -20℃, stable for up to one year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
E, WB
- 抗体英文名:
Cyclin O Antibody
- 抗体名:
Cyclin O Antibody
- 规格:
0.02mg
Cyclin O 抗体,Cyclin O Antibody,Cyclin O 抗体,Cyclin O Antibody
产品名称:Cyclin O 抗体-Cyclin O Antibody
产品货号:PSI-3859-0.02mg
产品规格:0.02mg
背景资料:Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.
保存建议:Cyclin O 抗体-Cyclin O Antibody stored at 4℃ for three months and -20℃, stable for up to one year
应用类型:E, WB
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文献和实验Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis
and members of the Bcl-2 protein family. In this chapter, we describe methods for measuring changes in Cdk2 activity during apoptosis. In addition, we also show the details of the generation of an antibody able to immunoprecipitate the cyclin O complexes
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies
, allergies, autoimmune diseases, and cancer. In this protocol we present a detailed method for enzymatic generation of disease-specific O- glycopeptides and how to monitor the antibody response to these in serum using microarray technology.
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