- ￥4500
- Sino Biological
- 北京
- 40592-V08H153
- 2025年08月12日
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- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-COV-2 hk.3 (Omicron) Spike RBD Protein (aa319-529, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
Coronavirus spike重组蛋白|Recombinant SARS-COV-2 hk.3 (Omicron) Spike RBD Protein (aa319-529, His Tag)（产品说明）
蛋白名称：SARS-COV-2 hk.3 (Omicron) Spike RBD Protein (aa319-529, His Tag)(HPLC-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (hk.3) Spike RBD (YP_009724390.1, with mutation G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, L455F, F456L, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H) (Arg319-Lys529) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant hk.3).
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 hk.3 (Omicron) Spike RBD Protein (aa319-529, His Tag) (Cat: 40592-V08H153), the EC50 is 1.2-3.6 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (hk.3) Spike RBD consists of 222 amino acids and predicts a molecular mass of 25.23 kDa. As a result of glycosylation, it migrates as an approximately 35.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-lys529
蛋白标签：C-His
蛋白名称：SARS-COV-2 hk.3 (Omicron) Spike RBD Protein (aa319-529, His Tag)(HPLC-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (hk.3) Spike RBD (YP_009724390.1, with mutation G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, L455F, F456L, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H) (Arg319-Lys529) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant hk.3).
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 hk.3 (Omicron) Spike RBD Protein (aa319-529, His Tag) (Cat: 40592-V08H153), the EC50 is 1.2-3.6 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (hk.3) Spike RBD consists of 222 amino acids and predicts a molecular mass of 25.23 kDa. As a result of glycosylation, it migrates as an approximately 35.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-lys529
蛋白标签：C-His
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文献和实验相关实验
日前，北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台，针对新型冠状病毒及其变异株，设计了编码新冠病毒刺突蛋白（Spike）受体结构域（RBD）的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗（circRNARBD
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Sino Biological Omicron Spike RBD重组蛋白 His标签 新冠S蛋白
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