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- 文献和实验
- 技术资料
- 品系:
原代细胞
- 库存:
999
- 英文名:
Human Hepatocytes
- 年限:
咨询销售
- 运输方式:
干冰
- 器官来源:
肝
- 物种来源:
人
- 组织来源:
人
- 规格:
2 x 10^6 cells/vial
人肝细胞说明:
哺乳动物的肝脏完成许多重要生理过程。不管在生理还是病理条件下,它都是能量代谢、外源性化学物质生物转化和血浆蛋白合成最重要的器官。原代培养人肝细胞是很好的实验模型,在过去和现在都是研究肝脏特定功能的重要工具。用繁殖的人肝细胞进行细胞移植、基因治疗和用于生物人工肝支持系统的肝细胞培养都在研究中。在合适的培养条件下,培养的人类肝细胞增殖并保持已分化的肝细胞功能,比如血清蛋白的合成。
ScienCell 研究实验室的 HH 是从人类肝脏中分离出来的。HH 纯化后立即冷冻保存并冷冻交付。每瓶 1 ml 体积中含有 >2 x 10^6 个细胞。HH 的特征是使用细胞角蛋白 18 和/或白蛋白特异性抗体进行免疫荧光。HH 对 HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。不建议将 HH 用于扩大或长期培养,因为细胞在培养中不会增殖。配套培养基: 肝细胞培养基(HM, Cat. No. 5201)。产品使用说明:仅供科研研究使用!
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文献和实验1.) Soronen, J., Yki?J�rvinen, H., Zhou, Y., S�devirta, S., Sarin, A., Leivonen, M., Sevastianova, K., Perttil�, J., Laurila, P., Sigruener, A., Schmitz, G. & Olkkonen, V.M. (2016) Novel hepatic microRNAs upregulated in human nonalcoholic fatty liver disease Physiol Rep. 4
2.) Yuan, J., Li, W., Huang, J., Guo, X., Li, X., Lu, X., Huang, X. & Zhang, H. (2015) Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane Stem Cell Res Ther. 6
3.) Xiao, H., Bid, H.K., Jou, D., Wu, X., Yu, W., Li, C., Houghton, P.J. & Lin, J. (2015) A Novel Small Molecular STAT3 Inhibitor, LY5, Inhibits Cell Viability, Cell Migration, and Angiogenesis in Medulloblastoma Cells* J Biol Chem. 290
4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
5.) Kong, L., Li, S., Huang, M., Xiong, Y., Zhang, Q., Ye, L., Liu, J., Zhu, X., Sun, R. & Guo, Y. (2015) The Roles of Endoplasmic Reticulum Overload Response Induced by HCV and NS4B Protein in Human Hepatocyte Viability and Virus Replication PLoS One. 10
6.) Feng, Y.M., Feng, C.W., Chen, S.Y., Hsieh, H.Y., Chen, Y.H. & Hsu, C.D. (2015) Cyproheptadine, an antihistaminic drug, inhibits proliferation of hepatocellular carcinoma cells by blocking cell cycle progression through the activation of P38 MAP kinase BMC Cancer. 15
7.) Chiang TS, Yang KC, Chiou LL, Huang GT, Lee HS.(2014) "Enhancement of CYP3A4 Activity in Hep G2 Cells by Lentiviral Transfection of Hepatocyte Nuclear Factor-1 Alpha." PloS one. 9: e94885.
8.) Liu Y, Liu A, Xu Z, Yu W, Wang H, Li C, Lin J. (2011) "XZH-5 inhibits STAT3 phosphorylation and causes apoptosis in human hepatocellular carcinoma cells." Apoptosis. 16: 502-10.
9.) Braconi C, Valeri N, Gasparini P, Huang N, Taccioli C, Nuovo G, Suzuki T, Croce CM, Patel T. (2010) "Hepatitis C virus proteins modulate microRNA expression and chemosensitivity in malignant hepatocytes." Clin Cancer Res. 16: 957-66.
10.) Hikita H, Takehara T, Shimizu S, Kodama T, Shigekawa M, Iwase K, Hosui A, Miyagi T, Tatsumi T, Ishida H, Li W, Kanto T, Hiramatsu N, Hayashi N. (2010) "The Bcl-xL inhibitor, ABT-737, efficiently induces apoptosis and suppresses growth of hepatoma cells in combination with sorafenib." Hepatology. 52: 1310-21.
11.) Liu Y, Li PK, Li C, Lin J. (2010) "Inhibition of STAT3 signaling blocks the anti-apoptotic activity of IL-6 in human liver cancer cells." J Biol Chem. 285: 27429-39.
12.) Shen Q, Cicinnati VR, Zhang X, Iacob S, Weber F, Sotiropoulos GC, Radtke A, Lu M, Paul A, Gerken G, Beckebaum S. (2010) "Role of microRNA-199a-5p and discoidin domain receptor 1 in human hepatocellular carcinoma invasion." Mol Cancer. 9: 227.
13.) Shimizu S, Takehara T, Hikita H, Kodama T, Miyagi T, Hosui A, Tatsumi T, Ishida H, Noda T, Nagano H, Doki Y, Mori M, Hayashi N. (2010) "The let-7 family of microRNAs inhibits Bcl-xL expression and potentiates sorafenib-induced apoptosis in human hepatocellular carcinoma." J Hepatol. 52: 698-704.
14.) Yeon JH, Na D, Park JK. (2010) "Hepatotoxicity assay using human hepatocytes trapped in microholes of a microfluidic device." Electrophoresis. 31: 3167-74.
15.) Braconi C, Meng F, Swenson E, Khrapenko L, Huang N, Patel T. (2009) "Candidate therapeutic agents for hepatocellular cancer can be identified from phenotype-associated gene expression signatures." Cancer. 115: 3738-48.
16.) Huang Y, Song N, Ding Y, Yuan S, Li X, Cai H, Shi H, Luo Y. (2009) "Pulmonary vascular destabilization in the premetastatic phase facilitates lung metastasis." Cancer Res. 69: 7529-37.
17.) Meng F, Henson R, Wehbe-Janek H, Ghoshal K, Jacob ST, Patel T. (2007) "MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer." Gastroenterology. 133: 647-58.
18.) Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. (2006) "Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes." Hepatology. 43: 257-65.
要封牢以免脱水和裂开。 E1270数据表上写着凝胶可稀释到2倍(diluted up to twofold)。这是什么意思? ECM Gel可用杜氏改良Eagle培养基(DMEM)稀释到2倍。意为ECM Gel可用等量的低温DMEM稀释。 这种凝胶加入细胞后会降解吗? 我们确知,本品在与细胞共培养7天后并没有降解。 我该如何从ECM Gel基质胶中回收细胞? 分散酶(D4818)是芽孢杆菌来源的中性蛋白酶,推荐用于ECM Gel基质胶(货号E1270)培养的细胞回收。分散酶的单细胞生产效率
一次做对!Erastin诱导HepG2细胞铁死亡实验的黄金操作手册请收好!
。 模型原理速览 Erastin作为经典的铁死亡诱导剂,通过抑制细胞膜上的胱氨酸/谷氨酸逆向转运体System Xc-(xCT),阻断胱氨酸摄取,导致谷胱甘肽(GSH)耗竭、谷胱甘肽过氧化物酶4(GPX4)失活,最终引发脂质过氧化累积和细胞死亡。HepG2细胞(人肝细胞癌细胞)对Erastin高度敏感,是体外模拟Erastin诱导铁死亡研究的理想模型。 所需试剂与材料 本次实验用到的核心试剂及对应作用整理如下,按需准备即可: 试剂名称 作用
www.westang.com 上海西唐生物科技公司 021-55229872 分装 ELISA 试剂盒 货号 品名 方法
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