- 询价
- ScienCell
- 5200-2
- 美国
- 2026年01月20日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
原代细胞
- 库存：
999
- 英文名：
Human Hepatocytes
- 年限：
咨询销售
- 运输方式：
干冰
- 器官来源：
肝
- 物种来源：
人
- 组织来源：
人
- 规格：
2 x 10^6 cells/vial
人肝细胞说明：
哺乳动物的肝脏完成许多重要生理过程。不管在生理还是病理条件下，它都是能量代谢、外源性化学物质生物转化和血浆蛋白合成最重要的器官。原代培养人肝细胞是很好的实验模型，在过去和现在都是研究肝脏特定功能的重要工具。用繁殖的人肝细胞进行细胞移植、基因治疗和用于生物人工肝支持系统的肝细胞培养都在研究中。在合适的培养条件下，培养的人类肝细胞增殖并保持已分化的肝细胞功能，比如血清蛋白的合成。
ScienCell 研究实验室的 HH 是从人类肝脏中分离出来的。HH 纯化后立即冷冻保存并冷冻交付。每瓶 1 ml 体积中含有 >2 x 10^6 个细胞。HH 的特征是使用细胞角蛋白 18 和/或白蛋白特异性抗体进行免疫荧光。HH 对 HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。不建议将 HH 用于扩大或长期培养，因为细胞在培养中不会增殖。配套培养基: 肝细胞培养基(HM, Cat. No. 5201)。产品使用说明：仅供科研研究使用！
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文献和实验1.) Soronen, J., Yki?J�rvinen, H., Zhou, Y., S�devirta, S., Sarin, A., Leivonen, M., Sevastianova, K., Perttil�, J., Laurila, P., Sigruener, A., Schmitz, G. & Olkkonen, V.M. (2016) Novel hepatic microRNAs upregulated in human nonalcoholic fatty liver disease Physiol Rep. 4
2.) Yuan, J., Li, W., Huang, J., Guo, X., Li, X., Lu, X., Huang, X. & Zhang, H. (2015) Transplantation of human adipose stem cell-derived hepatocyte-like cells with restricted localization to liver using acellular amniotic membrane Stem Cell Res Ther. 6
3.) Xiao, H., Bid, H.K., Jou, D., Wu, X., Yu, W., Li, C., Houghton, P.J. & Lin, J. (2015) A Novel Small Molecular STAT3 Inhibitor, LY5, Inhibits Cell Viability, Cell Migration, and Angiogenesis in Medulloblastoma Cells* J Biol Chem. 290
4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
5.) Kong, L., Li, S., Huang, M., Xiong, Y., Zhang, Q., Ye, L., Liu, J., Zhu, X., Sun, R. & Guo, Y. (2015) The Roles of Endoplasmic Reticulum Overload Response Induced by HCV and NS4B Protein in Human Hepatocyte Viability and Virus Replication PLoS One. 10
6.) Feng, Y.M., Feng, C.W., Chen, S.Y., Hsieh, H.Y., Chen, Y.H. & Hsu, C.D. (2015) Cyproheptadine, an antihistaminic drug, inhibits proliferation of hepatocellular carcinoma cells by blocking cell cycle progression through the activation of P38 MAP kinase BMC Cancer. 15
7.) Chiang TS, Yang KC, Chiou LL, Huang GT, Lee HS.(2014) "Enhancement of CYP3A4 Activity in Hep G2 Cells by Lentiviral Transfection of Hepatocyte Nuclear Factor-1 Alpha." PloS one. 9: e94885.
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15.) Braconi C, Meng F, Swenson E, Khrapenko L, Huang N, Patel T. (2009) "Candidate therapeutic agents for hepatocellular cancer can be identified from phenotype-associated gene expression signatures." Cancer. 115: 3738-48.
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18.) Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. (2006) "Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes." Hepatology. 43: 257-65.
要封牢以免脱水和裂开。 E1270数据表上写着凝胶可稀释到2倍(diluted up to twofold)。这是什么意思？ ECM Gel可用杜氏改良Eagle培养基(DMEM)稀释到2倍。意为ECM Gel可用等量的低温DMEM稀释。 这种凝胶加入细胞后会降解吗？ 我们确知，本品在与细胞共培养7天后并没有降解。 我该如何从ECM Gel基质胶中回收细胞？ 分散酶(D4818)是芽孢杆菌来源的中性蛋白酶，推荐用于ECM Gel基质胶（货号E1270）培养的细胞回收。分散酶的单细胞生产效率
www.westang.com 上海西唐生物科技公司 021-55229872 分装 ELISA 试剂盒 货号 品名 方法
