NAD/NADH检测试剂盒*SIGMA*MAK460-1KT

说明

一般描述

吡啶核苷酸在新陈代谢中起着重要作用，因此，人们对监测其浓度水平一直很感兴趣。NAD+/NADH 的定量测定可用于与细胞或组织的能量转化和氧化还原状态有关的研究。

Pyridine nucleotides, including nicotinamide adenine dinucleotide (NAD), are crucial in cellular metabolism, impacting how energy is processed and managed within cells. Monitoring the concentrations of NAD and its reduced form, NADH, is vital for understanding cellular energy dynamics and redox balance. NAD, functioning as a central electron carrier, alternates between NAD+ (oxidized) and NADH (reduced) forms, facilitating numerous metabolic reactions. This transformation is essential not only for redox reactions but also for other metabolic functions, such as ADP-ribosylation, which is crucial for regulating protein functions and DNA repair. Additionally, NAD+ acts as a substrate for sirtuins, which are important for energy metabolism and modulating oxidative stress. .

应用

 

Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study.: This study investigates the role of glycolysis in the myometrium of patients with adenomyosis, utilizing the NAD+/NADH Assay Kit to assess metabolic alterations (Huang et al., 2024). 10.1080/09513590.2024.2332411

 

Jian-Pi-Yi-Shen formula alleviates renal fibrosis by restoring NAD+ biosynthesis in vivo and in vitro.: This research demonstrates how the Jian-Pi-Yi-Shen formula mitigates renal fibrosis through NAD+ biosynthesis, with the NAD+/NADH Assay Kit playing a crucial role in the biochemical analysis (Gao et al., 2023). 10.18632/aging.205352

 

Butyrate inhibits the mitochondrial complex Ι to mediate mitochondria-dependent apoptosis of cervical cancer cells.: The study explores butyrate′s effects on mitochondrial function and apoptosis in cervical cancer cells, employing the NAD+/NADH Assay Kit to measure mitochondrial activity (Zhang et al., 2023). 10.1186/s12906-023-04043-3

 

PM2.5 mediates mouse testis Sertoli TM4 cell damage by reducing cellular NAD().: This article investigates the impact of PM2.5 on Sertoli cells in mouse testes, using the NAD+/NADH Assay Kit to track changes in cellular NAD levels (Xu et al., 2023). 10.1080/15376516.2023.2215862

 

Synergistic Effect and Mechanism of Plumbagin with Gentamicin Against Carbapenem-Resistant Klebsiella pneumoniae.: The research highlights the synergistic antibacterial effects of plumbagin and gentamicin, with the NAD+/NADH Assay Kit being used to evaluate the metabolic impact on bacterial cells (Chen et al., 2020). 10.2147/IDR.S265753

 

The NAD+/NADH Assay Kit may be used for:

Cancer Research

Neurodegenerative Research

Age/Geriatric Related Disease Research

 

特点和优势

Broad Detection Range: Detect NAD and NADH levels accurately across a wide range, from 0.02 to 1 μM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement

 

 

 

 

 

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 10 minute room temperature reaction, reducing complexity and saving valuable time and effort.

 

 

 

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

适用性

该试剂盒适用于测定NAD和NADH，以及评价药物对细胞、组织提取物等中NAD/NADH代谢的影响。

原理

NAD+/NADH 检测试剂盒基于乳酸脱氢酶循环反应，其中形成的 NADH 将探针还原为高荧光产物。在λEx=530 nm/λEm=585 nm处测得的该产品的荧光强度与样品中的NAD+/NADH浓度成正比。该测定对 NAD+/NADH 有高度D的特异性，NADP+/NADPH 的干扰最小 (<1%)，是一种测量 NAD、NADH 及其比率的便捷方法。

 

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