Lyn Protein, Human, Recombinan

蛋白长度：A DNA sequence encoding the human LYN isoform a (NP_002341.1) (Met 1-Pro 512) was fused with the GST tag at the N-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Met1-Pro512

标签类型：N-GST

分子量：The recombinant human LYN/GST chimera consists of 736 amino acids and predicts a molecular mass of 84.8 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.

活性：The specific activity was determined to be 30 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：LYN proto-oncogene, Src family tyrosine kinase

通用名：LYN

蛋白序参考：NP_002341.1

总结：Tyrosine-protein kinase Lyn is a member of the Src family of protein tyrosine kinases, which is mainly expressed in hematopoietic cells, in neural tissues liver, and adipose tissue. Tyrosine-protein kinase Lyn has many functions. Lyn kinase may downregulate the expression of stem cell growth factor receptor (KIT). Lyn kinase Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation. Lyn kinase also acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Lyn kinase relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. This kinase is involved in the induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. In a word, Lyn kinase functions primarily as a negative regulator, but can also function as an activator, depending on the context. Tyrosine-protein kinase Lyn is Required for the initiation of the B-cell response, but also its down-regulation and termination. It also plays an important role in the regulation of B-cell differentiation, proliferation, survival, and apoptosis, and is important for immune self-tolerance. It has been reported that Lyn kinase plays a role in the inflammatory response to bacterial lipopolysaccharide. Lyn kinase Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils, and eosinophils.

形式：Liquid

纯度：> 94 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM Reduced Glutathione, 10% glycerol, 0.5mM PMSF Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：JTK8 Protein, Human; p53Lyn Protein, Human; p56Lyn Protein, Human