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- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD45
- 级别：
CD45 Antibody
- 目录编号：
CD45 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
CD45 Antibody
- 抗体英文名：
Cyanine7 anti-human CD45 Antibody-PE
- 抗体名：
CD45 Antibody
- 标记物：
PE
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
CD45 Antibody
- 亚型：
CD45 Antibody
- 形态：
CD45 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
CD45 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥2500.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥5100.0 |
Description ：CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocytecommon antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasmamembrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signalingmolecule that regulates a variety of cellular processes including cell growth, differentiation, cellcycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigenreceptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, andother Src family kinases. CD45 non-covalently associates with lymphocyte phosphataseassociated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bindgalectin-1 and to be associated with several other cell surface antigens including CD1, CD2,CD3, and CD4.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Human PBMC
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 underoptimal conditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
4. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations：
1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
2. Carraro G, et al. 2021. Nat Med. 27:806. PubMed
3. Zhang XN, et al. 2021. Cell Res. . PubMed
4. Tu J, et al. 2022. Adv Sci (Weinh). 9:e2103631. PubMed
5. Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed
6. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
7. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
8. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
9. Zeng Y et al. 2019. Immunity. 51(5):930-948 . PubMed
10. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
11. Lin G, et al. 2021. Mol Med Rep. 23:. PubMed
12. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
#01 什么是同型对照？ 同型对照（Isotype Control），是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度，但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体与抗原的非特异性结合造成的背景染色。 #02 为什么要做同型对照？ 流式抗体与靶标的结合，是通过抗体的 Fab 端与靶标 Marker 进行特异性结合而完成的。白细胞（除T细胞外）表面大多表达 CD16、CD32、CD64 等 Fc 受体，这些受体可与各种抗体的 Fc 端非特异性结合，出现非特异
荧光标记基团的激发和发射波长是广大科研工作者最关心的内容.下面就我们大家常用的多肽标记的各种荧光基团数据参数提供给大家.荧光染料激发波长，nm发射波长，nmFITC4945185-FAM494522TAMRA560582Rhodamine B555580Cy3550570Cy5649670从发射波长相近的角度考虑,上述荧光基团在用AlexFluor替换时,可参照下表Alexa Fluor dyeAlternatives common dyesAlexa Fluor 350Coumarin
的抗体（待检标本）加到已知抗原标本上，在湿盒中37℃保温30min，使抗原抗体充分结合，然后洗涤，除去未结合的抗体。第二步，加上荧光标记的抗球蛋白抗体或抗IgG、IgM抗体。如果第一步发生了抗原抗体反应，标记的抗球蛋白抗体就会和已结合抗原的抗体进一步结合，从而可鉴定未知抗体。（二）试剂与仪器 磷酸盐缓冲盐水（PBS）：0.01mol/L，pH7.4 缓冲甘油：分析纯无荧光的甘油9份+ pH9.2 0.2M碳酸盐缓冲液1份配制。 荧光标记的抗人球蛋白抗体：以0.01mol/L，pH7.4的PBS
